DNA

Part:BBa_K201012:Design

Designed by: francesca ceroni   Group: iGEM09_Bologna   (2009-10-12)


Trans-Repressor (7) sequence, to be used for T-REX device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 80
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 81
    Illegal PstI site found at 95
    Illegal NotI site found at 7
    Illegal NotI site found at 88
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 81
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 81
    Illegal PstI site found at 95
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was designed by computer analysis



Source

Sequence has been synthesized by Sigma-Aldrich as single strand oligos, and annealed in our lab


References